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. Author manuscript; available in PMC: 2016 Feb 17.
Published in final edited form as: Anal Chem. 2015 Jan 27;87(4):2273–2281. doi: 10.1021/ac504118y

Figure 1.

Figure 1

Correct quantitation of ADP requires chromatographic separation. (A) The negative ionization mode-extracted ion chromatogram for U13C-ADP (+10, upper panel) and unlabeled ADP (lower panel). Yeast cells were grown in U13C-glucose, and metabolome was extracted with quenching solution spiked with unlabeled ADP. (B) The negative ionization mode-extracted ion chromatogram for the ADP channel in the ATP standard. The retention time of the “ADP” peak matched the retention time of the ATP standard, indicating that such a peak is an in-source fragment. (C) The negative ionization mode-extracted ion chromatogram for ADP and ATP channels in yeast cells grown on trehalose and 5 min after switching to glucose. Method A has been used throughout this figure.