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. Author manuscript; available in PMC: 2015 Mar 10.
Published in final edited form as: Methods Mol Biol. 2001;151:495–518. doi: 10.1385/1-59259-046-2:495

Table 1.

HYDROLYSIS OF FLUOROGENIC SUBSTRATES BY MMPS AND RELATED METALLOPROTEINASES

Substrate kcata KMa kcat/KMa Reference
(sec−1) (μM) (sec−1M−1)
MMP-1
Dnp-Pro-Gln-Gly~Ile-Ala-Gly-Trp ND ND 230b [Netzel-Arnett, 1991 #1307]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 827 [Murphy, 1992 #130]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 830 [Knight, 1992 #1292]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala~Nva-Trp-Met-Lys(Dnp)-NH2 (NFF-2) 0.15 142 1,060 [Nagase, 1994 #813]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 0.011c 7.1c 1,500c [Stack, 1989 #1290]
Mca-Pro-Lys-Pro-Gln-Gln~Phe-Phe-Gly~Leu-Lys(Dnp)-Gly (NFF-1) 0.04 16.6 2,510 [Nagase, 1994 #813]
Dnp-Pro-Leu-Ala~Tyr-Trp-Ala-Arg 0.13b 50b 2,700b [Netzel-Arnett, 1991 #1307]
Dnp-Pro-Leu-Ala~Leu-Trp-Ala-Arg 1.2b 130b 9,400b [Netzel-Arnett, 1991 #1307]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 12,100d [Knäuper, 1996 #1324]
Mca-Pro-Cha-Gly~Nva-His-Ala-Dpa-NH2 ND ND 14,700d [Knäuper, 1996 #1324]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 14,800 [Knight, 1992 #1292]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 2.5d 27.5d 92,200d [Neumann, 2004 #2671]
Dabcyl-Gaba-Pro-Gln-Gly~Leu-Glu(Edans)-Ala-Lys-NH2 ND ND 21,000 [Beekman, 1996 #902]
LY-Gly-Pro-Leu-Gly~Leu-Arg-Ala-Lys(Ctmr) ND 3.9 ND [Geoghegan, 1993 #1294]
Dns-Pro-Leu-Ala~Leu-Trp-Ala-Arg-NH2 0.417h 145h 7,300h [Holtz, 1999 #3301]
Dns-Pro-Leu-Ala~Cys(Mob)-Trp-Ala-Arg-NH2 0.150h 17 8,800h [Holtz, 1999 #3301]
Dnp-Pro-Cha-Gly~Cys(Me)-His-Ala-Lys(Nma)-NH2 ND ND 30,000 [Mohan, 2002 #2459]
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 76,000 [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 28,000 [Moss, 2007 #3292]
fTHP-4; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 0.06k 15.2k 3,900k [Minond, 2006 #2761]
MMP-1(Δ243–450)
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 653 [Murphy, 1992 #130]
fTHP-4; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 0.02k 13.3k 1,500k [Minond, 2006 #2761]
MMP-1 (19 kDa)
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 3,350b [Bickett, 1993 #1296]
Dnp-Pro-Cha-Gly~Cys(Me)-His-Ala-Lys(Nma)-NH2 ND ND 13,000b [Bickett, 1993 #1296]
MMP-1(1–242) + MMP-3(248–460)
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 511 [Murphy, 1992 #130]
MMP-2
Dnp-Pro-Gln-Gly~Ile-Ala-Gly-Trp ND ND 4,200b [Netzel-Arnett, 1991 #1307]
Dnp-Pro-Leu-Gly~Met-Trp-Ser-Arg 0.069b 7.8b 8,900b [Netzel-Arnett, 1991 #1307]
Mca-Pro-Lys-Pro-Gln-Gln~Phe-Phe-Gly-Leu-Lys(Dnp)-Gly (NFF-1) 1.07 100 10,800 [Nagase, 1994 #813]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala~Nva-Trp-Met-Lys(Dnp)-NH2 (NFF-2) 2.70 50 54,400 [Nagase, 1994 #813]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 58,000d [Knight, 1992 #1292]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 0.32c 2.6c 122,000c [Stack, 1989 #1290]
Dabcyl-Gaba-Pro-Gln-Gly~Leu-Glu(Edans)-Ala-Lys-NH2 ND ND 619,000 [Beekman, 1996 #902]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 629,000d [Knight, 1992 #1292]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 687,000d [Neumann, 2004 #2671]
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 29,000 [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 320,000 [Moss, 2007 #3292]
Dabcyl-His-Gly-Asp-Gln-Met-Ala-Gln-Lys-Ser-Lys(Fam)-NH2 ND ND 2,400 [Moss, 2007 #3292]
α1(V)436–447 fTHP; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Pro-Gly~Val-Val-Gly-Glu-Lys(Dnp)-Gly-Glu-Gln-(Gly-Pro-Hyp)5-NH2 0.062k 4.4k 14,000k [Lauer-Fields, 2003 #2028]
MMP-3
Dnp-Pro-Gln-Gly~Ile-Ala-Gly-Trp ND ND 61b [Netzel-Arnett, 1991 #1307]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 2,200 [Murphy, 1992 #130]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 2,200 [Knight, 1992 #1292]
Dnp-Pro-Tyr-Ala~Tyr-Trp-Met-Arg-NH2 0.24b 100b 2,400b [Netzel-Arnett, 1991 #1307]
Mca-Pro-Lys-Pro-Gln-Gln~Phe-Phe-Gly-Leu-Lys(Dnp)-Gly (NFF-1) 0.53 50 10,900 [Nagase, 1994 #813]
Dnp-Arg-Pro-Lys-Pro-Leu-Ala~Phe-Trp-NH2 ND ND 17,000d [Niedzwiecki, 1992 #768]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 23,000 [Knight, 1992 #1292]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 20,400d [Neumann, 2004 #2671]
Dabcyl-Gaba-Pro-Gln-Gly~Leu-Glu(Edans)-Ala-Lys-NH2 ND ND 40,000 [Beekman, 1996 #902]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala~Nva-Trp-Met-Lys(Dnp)-NH2 (NFF-2) 3.95 66 59,400 [Nagase, 1994 #813]
Dnp-Arg-Pro-Lys-Pro-Leu-Ala~Nva-Trp-NH2 ND ND 45,000d [Niedzwiecki, 1992 #768]
Mca-Arg-Pro-Lys-Pro-Val-Glu~Nva-Trp-Arg-Lys(Dnp)-NH2 (NFF-3) 1.31d 20d 65,700d [Nagase, 1994 #813]
Mca-Arg-Pro-Lys-Pro-Val-Glu~Nva-Trp-Arg-Lys(Dnp)-NH2 (NFF-3) 5.40 25 218,000 [Nagase, 1994 #813]
Mca-Arg-Pro-Lys-Pro-Val-Glu~Nva-Trp-Arg-Lys(Dnp)-NH2 (NFF-3) 2.20e 7.5e 302,000e [Nagase, 1994 #813]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 6,500d [Park, 2000 #1692]
Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dpa-Ala-Arg-NH2 ND ND 45,300d [Park, 2000 #1692]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 ND ND 20,700d [Park, 2000 #1692]
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 ND ND 80,200d [Park, 2000 #1692]
Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 ND ND 1,200d [Park, 2000 #1692]
Dnp-Pro-Cha-Gly~Cys(Me)-His-Ala-Lys(Nma)-NH2 ND ND 500 [Mohan, 2002 #2459]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 4,000 [Moss, 2007 #3292]
MMP-3(Δ248–460)
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 2,390 [Murphy, 1992 #130]
MMP-3(1–233) + MMP-1(229–450)
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 2,750 [Murphy, 1992 #130]
MMP-7
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 11,700 [Knight, 1992 #1292]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 13,000f [Crabbe, 1992 #3294]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 169,000 [Knight, 1992 #1292]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 295,000d [Neumann, 2004 #2671]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 131,000d [Park, 2000 #1692]
Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dpa-Ala-Arg-NH2 ND ND 320,000d [Park, 2000 #1692]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 ND ND 28,300d [Park, 2000 #1692]
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 ND ND 5,620d [Park, 2000 #1692]
Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 ND ND 3,420d [Park, 2000 #1692]
Dns-Pro-Leu-Ala~Leu-Trp-Ala-Arg-NH2 3.5h 114h 23,000h [Holtz, 1999 #3301]
Dns-Pro-Leu-Ala~Cys(Mob)-Trp-Ala-Arg-NH2 0.090h 20h 5,000h [Holtz, 1999 #3301]
Dnp-Pro-Cha-Gly~Cys(Me)-His-Ala-Lys(Nma)-NH2 ND ND 10,000i [Mohan, 2002 #2459]
MMP-8
Dnp-Pro-Gln-Gly~Ile-Ala-Gly-Trp 21.0b 1100b 19,000b [Netzel-Arnett, 1991 #1307]
Dnp-Pro-Leu-Ala~Leu-Trp-Ala-Arg 9.4b 50b 190,000b [Netzel-Arnett, 1991 #1307]
Dnp-Pro-Leu-Ala~Tyr-Trp-Ala-Arg 3.1b 7.7b 400,000b [Netzel-Arnett, 1991 #1307]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 418,000d [Neumann, 2004 #2671]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 140,000 [Moss, 2007 #3292]
fTHP-4; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 0.035k 7.6k 4,500k [Minond, 2006 #2761]
C10-fTHP-4 0.089k 14.4k 6,200k [Minond, 2006 #2761]
C16-fTHP-4 0.016k 9.95k 1,600k [Minond, 2006 #2761]
fTHP-9; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.94k 5.4k 175,000k [Minond, 2006 #2761]
C10-fTHP-9 0.64k 8.8k 73,000k [Minond, 2006 #2761]
C16-fTHP-9 0.13k 9.6k 14,000k [Minond, 2006 #2761]
fTHP-10; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Orn-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.035k 8.15k 4,300k [Minond, 2006 #2761]
fTHP-11; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Orn-Gly~Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.38k 19.7k 19,000k [Minond, 2006 #2761]
C10-fTHP-11 0.007k 3.60k 1,900k [Minond, 2006 #2761]
C16-fTHP-11 0.007k 3.50k 2,100k [Minond, 2006 #2761]
C6-fTHP-12; C6-(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Leu-Gly~Met-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.070k 14.5k 5,090k [Minond, 2007 #2768]
fTHP-13; Cn-(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Val-Asn~Phe-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.028k 9.30k 3,010k [Minond, 2007 #2768]
C6-fTHP-13 0.020k 3.90k 3,870k [Minond, 2007 #2768]
MMP-8 (Met80 or Leu81 N-terminus)
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 135,000e [Knäuper, 1996 #1324]
Mca-Pro-Cha-Gly~Nva-His-Ala-Dpa-NH2 ND ND 166,000e [Knäuper, 1996 #1324]
MMP-8 (Phe79 N-terminus)
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 193,000d [Knäuper, 1996 #1324]
Mca-Pro-Cha-Gly~Nva-His-Ala-Dpa-NH2 ND ND 252,000d [Knäuper, 1996 #1324]
MMP-9
Dnp-Pro-Gln-Gly~Ile-Ala-Gly-Trp ND ND 1,200b [Netzel-Arnett, 1991 #1307]
Mca-Arg-Pro-Lys-Pro-Val-Glu~Nva-Trp-Arg-Lys(Dnp)-NH2 (NFF-3) 0.282 28 10,100 [Nagase, 1994 #813]
Dnp-Pro-Leu-Gly~Met-Trp-Ser-Arg 0.069b 5.2b 13,000b [Netzel-Arnett, 1991 #1307]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala~Nva-Trp-Met-Lys(Dnp)-NH2 (NFF-2) 1.38 25 55,300 [Nagase, 1994 #813]
Dnp-Pro-Leu-Gly~Leu-Trp-Ala-D-Arg-NH2 ND ND 69,400b [Bickett, 1993 #1296]
Dnp-Pro-Cha-Gly~Cys(Me)-His-Ala-Lys(Nma)-NH2 ND ND 86,600b [Bickett, 1993 #1296]
Dnp-Pro-Cha-Gly~Cys(Me)-His-Ala-Lys(Nma)-NH2 ND ND 300,000 [Mohan, 2002 #2459]
Dabcyl-Gaba-Pro-Gln-Gly~Leu-Glu(Edans)-Ala-Lys-NH2 ND ND 206,000 [Beekman, 1996 #902]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 169,000 [Knight, 1992 #1292]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 623,000d [Neumann, 2004 #2671]
Abz-Gly-Pro-Leu-Gly~Leu-Tyr(NO2)-Ala-Arg-NH2 ND ND 150,000 [Buchardt, 1999 #1847]
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 850,000 [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 220,000 [Moss, 2007 #3292]
α1(V)436–447 fTHP; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Pro-Gly~Val-Val-Gly-Glu-Lys(Dnp)-Gly-Glu-Gln-(Gly-Pro-Hyp)5-NH2 0.044k 8.1k 5,500k [Lauer-Fields, 2003 #2028]
MMP-11
Dns-Pro-Leu-Ala~Leu-Trp-Ala-Arg-NH2 0.012h 21h 570h [Holtz, 1999 #3301]
Dns-Pro-Leu-Ala~Cys(Mob)-Trp-Ala-Arg-NH2 0.078h 5.2h 15,000h [Holtz, 1999 #3301]
MMP-12
Dnp-Arg-Pro-Leu-Ala-Leu~Trp-Arg-Ser-NH2 ND ND 18,000 [Gronski, 1997 #3300]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 61,000d [Park, 2000 #1692]
Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dpa-Ala-Arg-NH2 ND ND 968,000d [Park, 2000 #1692]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 ND ND 243,000d [Park, 2000 #1692]
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 ND ND 83,300d [Park, 2000 #1692]
Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 ND ND 13,000d [Park, 2000 #1692]
α1(V)436–447 fTHP; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Pro-Gly~Val-Val-Gly-Glu-Lys(Dnp)-Gly-Glu-Gln-(Gly-Pro-Hyp)5-NH2 2.35k 24.8k 97,600k [Bhaskaran, 2008 #3295]
MMP-13
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 757,000d [Knäuper, 1996 #1324]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 5.9d 5.2d 1,135,000d [Neumann, 2004 #2671]
Mca-Pro-Cha-Gly~Nva-His-Ala-Dpa-NH2 ND ND 1,090,000d [Knäuper, 1996 #1324]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 2,520d [Park, 2000 #1692]
Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dpa-Ala-Arg-NH2 ND ND 2,680d [Park, 2000 #1692]
Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 ND ND 408d [Park, 2000 #1692]
Dabcyl-Gly-Pro-Leu-Gly-Met-Arg-Gly-Cys(fluorescein)-NH2 ND ND 1,600,000d [Rasmussen, 2004 #2700]
Dabcyl-Gly-Pro-Leu-Gly-Phe-Arg-Val-Cys(fluorescein)-NH2 ND ND 2,700,000d [Rasmussen, 2004 #2700]
Dabcyl-Gly-Pro-Ser-Gly-Ile-His-Val-Cys(fluorescein)-NH2 ND ND 2,500,000d [Rasmussen, 2004 #2700]
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 2,100,000 [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 460,000 [Moss, 2007 #3292]
fTHP-4; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 0.015k 8.6k 1,600k [Minond, 2006 #2761]
C10-fTHP-4 0.013k 3.4k 3,800k [Minond, 2006 #2761]
C16-fTHP-4 0.0020k 1.4k 1,300k [Minond, 2006 #2761]
fTHP-9; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.16k 6.25k 25,000k [Minond, 2006 #2761]
C10-fTHP-9 0.060k 3.45k 18,000k [Minond, 2006 #2761]
C16-fTHP-9 0.028k 2.50k 11,000k [Minond, 2006 #2761]
fTHP-10; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Orn-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.58k 30.1k 19,000k [Minond, 2006 #2761]
C10-fTHP-10 0.31k 17.4k 18,000k [Minond, 2006 #2761]
fTHP-11; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Orn-Gly~Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.18k 13.7k 13,000k [Minond, 2006 #2761]
C10-fTHP-11 0.013k 7.10k 1,900k [Minond, 2006 #2761]
C16-fTHP-11 0.007k 7.68k 940k [Minond, 2006 #2761]
C6-fTHP-12; C6-(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Leu-Gly~Met-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.060k 6.35k 9,710k [Minond, 2007 #2768]
fTHP-13; Cn-(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Val-Asn~Phe-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.127k 20.5k 6,116k [Minond, 2007 #2768]
C6-fTHP-13 0.060k 9.95k 5,800k [Minond, 2007 #2768]
MMP-13(Δ249–451)
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 639,000d [Knäuper, 1997 #1060]
Mca-Pro-Cha-Gly~Nva-His-Ala-Dpa-NH2 ND ND 1,000,000d [Knäuper, 1997 #1060]
MMP-14 catalytic domain
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 393,000d [Neumann, 2004 #2671]
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 9.9 7.9 1,253,000d [Neumann, 2004 #2671]
Dns-Pro-Leu-Ala~Leu-Trp-Ala-Arg-NH2 0.67h 27h 25,000h [Holtz, 1999 #3301]
Dns-Pro-Leu-Ala~Cys(Mob)-Trp-Ala-Arg-NH2 5.6h 8h 700,000h [Holtz, 1999 #3301]
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 1,900 [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 73,000 [Moss, 2007 #3292]
MMP-14(Δ279–523)
fTHP-4; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 1.37k 23.1k 59,000k [Minond, 2006 #2761]
C10-fTHP-4 0.0033k 0.80k 4,100k [Minond, 2006 #2761]
C16-fTHP-4 0.0021k 1.80k 1,200k [Minond, 2006 #2761]
fTHP-9; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 1.34k 7.95k 168,000k [Minond, 2006 #2761]
C10-fTHP-9 0.080k 2.80k 29,000k [Minond, 2006 #2761]
C16-fTHP-9 0.200k 6.60k 30,000k [Minond, 2006 #2761]
fTHP-10; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Orn-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.25k 10.2k 25,000k [Minond, 2006 #2761]
C10-fTHP-10 0.091k 4.40k 20,000k [Minond, 2006 #2761]
fTHP-11; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Orn-Gly~Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 1.30k 16.6k 78,000k [Minond, 2006 #2761]
C10-fTHP-11 0.036k 3.50k 11,000k [Minond, 2006 #2761]
MMP-14(Δ319–523)
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 73,700d [Hurst, 2004 #2356]
fTHP-4; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 ND ND 51,200d [Hurst, 2004 #2356]
MMP-14
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 14,600d [Hurst, 2004 #2356]
fTHP-4; Cn-(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly~Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 ND ND 21,800d [Hurst, 2004 #2356]
MMP-15(Δ268–628)
fTHP-11; (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Orn-Gly~Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2l 0.41k 14.3k 29,000k [Minond, 2006 #2761]
MMP-17 catalytic domain
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 0.05d 8d 5,800d [Wang, 1999 #3299]
Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 0.42d 26d 16,000d [Wang, 1999 #3299]
MMP-25 catalytic domain
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 38,000d [English, 2001 #2760]
Mca-Pro-Leu-Ala-Nva-Dpa-Ala-Arg-NH2 ND ND 83,000d [English, 2001 #2760]
MMP-26
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 1,850d [Park, 2000 #1692]
Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dpa-Ala-Arg-NH2 ND ND 343d [Park, 2000 #1692]
Dnp-Pro-Leu-Gly~Met-Trp-Ser-Arg ND ND 9,400d [Park, 2002 #3298]
Mca-Pro-Cha-Gly~Nva-His-Ala-Dpa-NH2 ND ND 17,000d [Park, 2002 #3298]
Mca-Pro-Leu-Ala~Nva-Dpa-Ala-Arg-NH2 ND ND 30,000d [Park, 2002 #3298]
Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 22,000d [Park, 2002 #3298]
ADAM8
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 2,600j [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 100,000j [Moss, 2007 #3292]
Dabcyl-His-Gly-Asp-Gln-Met-Ala-Gln-Lys-Ser-Lys(Fam)-NH2 ND ND 53,000j [Moss, 2007 #3292]
ADAM10
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 56j [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 6,200j [Moss, 2007 #3292]
Dabcyl-His-Gly-Asp-Gln-Met-Ala-Gln-Lys-Ser-Lys(Fam)-NH2 ND ND 270j [Moss, 2007 #3292]
ADAM12
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 3,000j [Moss, 2007 #3292]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 280,000j [Moss, 2007 #3292]
Dabcyl-His-Gly-Asp-Gln-Met-Ala-Gln-Lys-Ser-Lys(Fam)-NH2 ND ND 40j [Moss, 2007 #3292]
ADAM17 (TACE)
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2 ND ND 778,400d [Neumann, 2004 #2671]
Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Ala-Arg-NH2 ND ND 363,800d [Neumann, 2004 #2671]
Dabcyl-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Ala-Arg-NH2 ND ND 466,300d [Neumann, 2004 #2671]
Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2 ND ND 430,000j [Moss, 2007 #3292]
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 ND ND 6,800j [Moss, 2007 #3292]
ADAMTS-4-1
Fam-Ala-Glu~Leu-Gln-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-Tamra 0.86g 10.7g 80,200g [Wayne, 2007 #3068]
Lys(6-Fam)-Asp-Val-Gln-Glu-Phe-Arg-Gly-Val-Thr-Ala-Val-Ile-Arg-Cys(Qsy-9)-Lys-Gly-Lys 0.22 1.0 215,000 [Hills, 2007 #3042]
ADAMTS-4-2
fTHPa 0.0120d 47.1d 415d [Lauer-Fields, 2007 #2649]
fSSPa 0.0720d 279.0d 258d [Lauer-Fields, 2007 #2649]
Fam-Ala-Glu~Leu-Gln-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-Tamra 2.79g 16.2g 172,000g [Wayne, 2007 #3068]
a

Assays were performed at 37 °C, pH 7.5 except where noted. ND = not determined.

b

Assay performed at 23 °C.

c

Porcine, not human, MMP.

d

Assay performed at 25 °C.

e

Assay performed at pH 6.0.

f

Assay performed at pH 6.5.

g

Assay performed at 20 °C, pH 8.0.

h

Assay performed at 25 °C, pH 6.8.

i

Assay performed at 22 °C.

j

Assay performed at 25 °C, pH 8.0.

k

Assay performed at 30 °C.

l

Substitutions relative to fTHP-4 are indicated in bold.