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. 2014 Sep 30;40(2):78–88. doi: 10.1503/jpn.130285

Fig. 1.

Fig. 1

Phospholipase C-β1 (PLC-β1) knockdown in the medial prefrontal cortex (mPFC) by injection of small hairpin RNA (shRNA) lenti-virus. (A) PLC-β1-specific shRNA vectors reduced endogenous PLC-β1 expression in C2C12 cell cultures. An antibody against β-actin was used as an internal control for each sample. Lane C is a negative control. (B) Lentiviral vectors expressing shRNA for PLC-β1 (shPLC-β1) or a scrambled sequence (shSCR) were bilaterally injected into the mPFC of a mouse brain (right panel). Green fluorescent protein (GFP) expression under the control of the synapsin-1 promoter was detected in the mPFC (left panel). (C) Immunofluorescence staining for PLC-β1 expression in the mPFC of mice injected with shSCR (panels 1–8) and shPLC-β1 (panels 9–16). Panels 5–8 and 13–16 are higher magnification images corresponding to the rectangles in panels 1–4 and 9–12, respectively. *PLCβ-1-positive neurons in mice injected with shSCR. Scale bars: 100 μm (panels 1–4, 9–12) and 18.8 μm (panels 5–8, 13–16). DAPI = diamidino-2-phenylindole.