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. Author manuscript; available in PMC: 2015 Jul 8.
Published in final edited form as: Nature. 2014 Oct 22;517(7533):205–208. doi: 10.1038/nature13828

Extended Data Figure 8. C. difficile growth inhibition by secondary bile acids and intestinal content from antibiotic-naive animals.

Extended Data Figure 8

Addition of the secondary bile acids deoxycholate (DCA) (a) or lithochoate (LCA) (b) to culture media inhibits C. difficile. Bile acid-dependent inhibition of C. difficile enumerated by recovery of CFU after inoculation of vegetative C. difficile into cell-free (c) or whole (d) intestinal content harvested from C57BL/6J mice (n=5–6 per group), with or without pre-incubation with cholestyramine. **P<0.01, Mann-Whitney (two-tailed) (c,d).