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. 2015 Mar 10;9(3):e0003601. doi: 10.1371/journal.pntd.0003601

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© 2015 Falcão et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — Bone-marrow neutrophils were co-cultured with L. braziliensis. After 18h, neutrophils were labeled with Annexin V and DAPI and analyzed by FACS. (A) Representative zebra plots of neutrophils (PMN), neutrophils cultured with L. braziliensis (PMN+Lb) or neutrophils exposed to UV (UV). (B) Percentage of DAPI⁺/Annexin V⁺ neutrophils (PMN) or neutrophils cultivated with L. braziliensis (PMN+Lb). (C) Representative zebra plots of bystander neutrophils (mCherry-) and of infected neutrophils (mCherry⁺) expressing DAPI and Annexin V. (D) Representative dot plots of neutrophils infected with mCherry L. braziliensis after 18h, bar graph represents the percentage of non-infected (mCherry^-) and infected (mCherry⁺) neutrophils. Data (mean ± SEM) are from one experiment representative of three experiments. **p <0.01.