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. 2015 Mar 4;9(3):e0003546. doi: 10.1371/journal.pntd.0003546

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Fig 6 — Absolute number of Ly-6G⁺ neutrophils shows in (A) C57BL/6 and (B) BALB/c mice ears. Absolute number of dermal IL-17⁺ Ly-6G⁺ neutrophils determined by flow cytometry in (C) C57BL/6 and (D) BALB/c mice. Data were collected at day 2, 42 (C57BL/6) and 70 (BALB/c) post injection. Recovered ear cells were retimulated with 25 μg/mL soluble Leishmania antigen, 5 ng/mL IL-2 and 10 μg/mL anti-CD28 overnight before performing cytokine staining for IL-17. Each data set was collected from two experiments with similar results. Values represent mean ± SD. n = 3 mice. Significant differences were determined by Student t-test. *p < 0.05.