Figure 5.

Leptin regulation of Pdyn neurons is required for normal energy homeostasis in DIO mice. Male LepRb^PdynKO (KO) and littermate controls (C) were placed on chow or high fat diets, and body weight measured weekly (A) (mean ± SEM is shown; *p < 0.05 by ANOVA). At 12–14 weeks of age, animals underwent body composition analysis by NMR spectroscopy (B). Serum from HFD-fed control and LepRb^PdynKO mice was assayed for leptin (C) or insulin (D). Blood glucose was measured following a 24 h fast (E) in the HFD cohort. (B–E) mean ± SEM is shown; *p < 0.05 by t-test. (F–H) A subset of HFD-fed control and LepRb^PdynKO mice were assessed in CLAMS metabolic cages for 3 days following body composition analysis. Food intake (F) was measured on the final day. VO₂ was also measured and is presented normalized to total body mass (G) and lean body mass (H) (Mean ± SEM is shown; ***p < .0001 by ANOVA). HFD, high fat diet (Research diets, 60% kcal from fat). Black triangles = HFD fed LepRb^PdynKO; white triangles = HFD fed controls; black circles = chow fed LepRb^PdynKO; white circles = chow fed controls. Black bars = LepRb^PdynKO; white bars = controls. N = 8–14 for chow and HFD cohorts. N = 6–7 animals per genotype for VO₂ measurements.