Table 1.
List of Clic4-binding proteins identified by mass-spectrometry. Proteins co-immunoprecipitated with Clic4 from βTC-tet cells (Figure 7) were identified by mass spectrometry. Most of the proteins were pulled-down with the C-terminal antibody. *Indicates the proteins pulled-down with the N-terminal antibody and ** indicates those pulled-down with both C- and N-terminal antibodies.
| List of Clic4-binding proteins | |||
|---|---|---|---|
| Proteins involved in protein biosynthesis | Proteins involved in cell cycle | ||
| 1 | Ile-tRNA synthetase | 46 | DNA replication licensing factor MCM2 |
| 2 | Arg-tRNA synthetase | 47 | Replication factor C subunit 5 |
| 3 | Met-tRNA synthetase | 48 | Chromosome transmission fidelity protein 18 homolog |
| 4 | Leu-tRNA synthetase | 49 | Paired amphipathic helix protein Sin3a |
| 5 | Asp-tRNA synthetase | 50 | CD2-associated protein |
| 6 | Lys-tRNA synthetase | 51 | Sister chromatid cohesion protein DCC1 |
| 7 | Ala-tRNA synthetase | 52* | Proliferating cell nuclear antigen |
| 8 | Thr-tRNA synthetase | 53 | Centrosomal protein of 89 kDa |
| 9 | Glu-Pro-tRNA synthetase | 54 | Kinetochore-associated protein NSL1 homolog |
| 10 | AIMP1/p43 | 55 | Kinetochore-associated protein DSN1 homolog |
| 11 | AIMP2/p38 | 56 | Polyamine-modulated factor 1 |
| 12 | AIMP3/p18 | 57 | Protein CASC5 |
| 13–17 | eIF3 subunit B, A, H, M, E | 58* | NIF3-like protein 1 |
| 18** | 40S ribosomal protein S9 | 59* | Cyclin-G-associated kinase |
| 19** | 60S ribosomal protein L17 | Kinases/Phosphatases | |
| Proteins involved in apoptosis | 60-61 | cAMP-dependent protein kinase type I-alpha and type II-alpha. | |
| 10 | AIMP1/p43 | 62* | Serine/threonine-protein kinase MRCK beta |
| 11 | AIMP2/p38 | 63* | Protein phosphatase 1 regulatory subunit 12A |
| 12 | AIMP3/p18 | 64* | Myotubularin-related protein 5 |
| Proteins involved in protein degradation | 65* | Serine/threonine-protein phosphatase PP1-beta catalytic subunit | |
| 20–26 | 26S proteasome non-ATPase regulatory subunit 2, 1, 11, 13, 6, 7, 5. | 66 | Type II inositol 1,4,5-trisphosphate 5-phosphatase |
| 27–31 | 26S protease regulatory subunit 8, 6B, 7, 10B, 4. | Proteins involved in GTPase activity | |
| 32 | F-box only protein 28 | 67 | Arf-GAP |
| 33 | DNA damage-binding protein 1 | 68 | RhoGEF (Factor 12 and 1) |
| Proteins transport | 69 | Dedicator of cytokinesis protein 7 | |
| 34 | Kinesin-1 heavy chain | 70 | SH3 domain-binding protein 1 |
| 35–37 | Kinesin light chain 1, 2 and 4 | 71* | Neurofibromin |
| 38 | Kinesin-like protein KIF11 | 72* | BTB/POZ domain-containing protein KCTD12 |
| 39 | 14-3-3 protein epsilon | 73* | GTPase-activating protein and VPS9 domain-containing protein 1 |
| 40 | Torsin-1A-interacting protein 1 | 74* | Jouberin |
| 41 | AP-3 complex subunit beta-1 | Others | |
| 42* | Membrane-associated phosphatidylinositol transfer protein 1 | 43* | SUN domain-containing protein 2 |
| 75 | Spectrin beta chain | 76 | Spectrin alpha chain |
| 44 | Myomegalin | 77 | F-actin-capping protein subunit beta |
| Proteins involved in cell cycle | 78* | 3-hydroxyisobutyrate dehydrogenase, mitochondrial | |
| 45 | DNA replication licensing factor MCM3 | ||
Unmarked, protein pull-down with C-ter Ab; *, protein pull-down with N-ter Ab; **, protein pull down by both C- and N-ter Ab.