Figure 3.

Reduced β-cell mass, Pdx-1 and Nkx6.1 positive islet cells in βTCFDN offspring when pregnant mothers were fed with doxycycline to induce TCF7L2DN expression. A–B) Assessment of β-cell mass (A) and α-cell (B) mass. n = 3. C–D) Immunostaining shows reduced percentage of Pdx-1 positive islet cells in βTCFDN mice. Immunostaining shows reduced percentage of Nkx6.1 positive islet cells in βTCFDN mice. The percentages were determined by counting 2,200–3,000 islet cells from βTCFDN mice and the Ins2-rtTA control mice (n = 3 for each group).