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Application of a multilayered heparin gel microstructure (H/H) for hepatocyte-3T3 cell co-cultures. A) and B) Bright-field microscopic images of co-culture at day 1 and 9. Hepatocyte were cultured on inside microwells and 3T3 fibroblasts were cultured on the wall around microwells. C) Intracellular albumin of hepatocytes co-cultured on all heparin gel microwells (H/H) at day 9. D) ELISA analysis of albumin secretion by hepatocyte co-cultured on H/H during 9 days.
