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. Author manuscript; available in PMC: 2016 Mar 4.
Published in final edited form as: J Am Chem Soc. 2015 Feb 18;137(8):3004–3016. doi: 10.1021/ja512204s

Figure 10.

Figure 10

4-pulse ESEEM frequency domain of 0.18:1 Mn(II):15N-CP-Ser (red) and Mn(II)- and Ca(II)-bound to 15N-CP-Ser. The concentration of Mn(II) in both spectra is 180 μM. Spectrometer settings: νmw = 34.1 GHz, B0 ~ 1240 mT, π/2 = 8 ns, τ = 200 ns, T0 = 80 ns, temperature = 10 K. Protein sample conditions: 1 mM CP-Ser (–Ca) or 200 μM CP-Ser (+Ca), 180 μM Mn(II), pH 7.5 (75 mM HEPES, 100 mM NaCl, 20% (v/v) PEG 200, ± 2 mM Ca(II).