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. Author manuscript; available in PMC: 2016 Mar 4.
Published in final edited form as: J Am Chem Soc. 2015 Feb 18;137(8):3004–3016. doi: 10.1021/ja512204s

Figure 12.

Figure 12

Exchangeable proton counting of Mn(II)-bound CP-Ser with varying ratios of Mn / CP. Spectrometer settings: νmw = 9.525(5) GHz, B0 = 327.35 mT, π/2 = 8 ns, τ = 216 ns, T0 = 80 ns, temperature = 10 K. Protein sample conditions: 1 mM CP-Ser (red) or 200 μM CP-Ser (blue), 180 μM Mn(II), pH 7.5 (75 mM HEPES, 100 mM NaCl, 20% (v/v) PEG 200).