Table 1.
Primer sequences used in multiplex PCR for detection of pathogenic E. coli.
| E. coli type | Primer sequences | Product size in bp | References |
|---|---|---|---|
| ETEC (LT) | |||
| ETEC 508F | 5′-CACACGGAGCTCCTCAGTC-3′ | 508 bp | [23] |
| ETEC 508R | 5′-CCCCCAGCCTAGCTTAGTTT-3′ | ||
| ETEC (ST) | |||
| ETEC 147F | 5′-GCTAAACCAGTAGAGGTCTTCAAAA-3′ | 147 bp | [23] |
| ETEC 147R | 5′-CCCGGTACAGAGCAGGATTACAACA-3′ | ||
| EHEC (Stx1) | |||
| EHEC 384F | 5′-CAGTTAATGTGGTGGCGAAGG-3′ | 384 bp | [23] |
| EHEC 384R | 5′-CACCAGACAATGTAACCGCTG-3′ | ||
| EHEC (Stx2) | |||
| EHEC 584F | 5′-ATCCTATTCCCGGGAGTTACG-3′ | 584 bp | [23] |
| EHEC 584R | 5′-GCGTCATCGTATACACAGGAGC-3′ | ||
| EPEC (eae) | |||
| EPEC 881F | 5′-CCCGAATTCGGCACAAGCATAAGC-3′ | 881 bp | [23] |
| EPEC 881R | 5′-CCCGGATCCGTCTCGCCAGTATTCG-3′ | ||
| EPEC (bfpA) | |||
| EPEC 300F | 5′-GGAAGTCAAATTCATGGGGGTAT-3′ | 300 bp | [23] |
| EPEC 300R | 5′-GGAATCAGACGCAGACTGGTAGT-3′ | ||
| EIEC (ipaH) | |||
| EIEC 423F | 5′-TGGAAAAACTCAGTGCCTCT-3′ | 423 bp | [23] |
| EIEC 423R | 5′-CCAGTCCGTAAATTCATTCT-3′ | ||
| EAEC (aatA) | |||
| EAEC 650F | 5′-CTGGCGAAAGACTGTATCAT-3′ | 650 bp | [23] |
| EAEC 650R | 5′-CAATGTATAGAAATCCGCTGTT-3′ | ||
| EAEC (aaic) | |||
| EAEC 215F | 5′-ATTGTCCTCAGGCATTTCAC-3′ | 215 bp | [23] |
| EAEC 215R | 5′-ACGACACCCCTGATAAACAA-3′ |