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. 2015 Feb 24;2015:620364. doi: 10.1155/2015/620364

Figure 3.

Figure 3

Longitudinal follow-up of the fate of LinBMCs at different time points post intravitreal transplantation. Immunofluorescence analysis of retinas after intravitreal GFP+LinBMCs delivery showed transplanted cells (green) on the 7th day (a, b) and at 3 months after injection (c). Quantitative analysis of the total number of incorporated LinBMCs per eye section (n = 5/time point) (d). Coreactivity of GFP (green) and Isolectin-B4 (red) by fluorescent lectin staining showed that transplanted GFP+LinBMCs locally differentiated into Isolectin-B4-positive macrophages (inserts) in LinBMC-injected eyes on the 7th day after transplantation (e) and at 3 months after injection (f). Scale bar = 20 μm. * P < 0.05 for day 7 versus other time points.