Table 1.

Single-Nucleotide Polymorphisms Identified in Whole-Genome Sequencing of Parasites Resistant to ML238

Chromosome	Position	Dd2		ML238R		Gene ID	Annotation	Coding Position	Protein Position	Dd2		ML238R		BRD6323R
		Base	Quality	Base	Quality					Codon	a.a.	Codona	a.a.	Codonb	a.a.
M76611	3589	G	93	C	93	mal_mito_3	Cytb	98	33	GGA	G	GCA	A	GTA	V
Pf3D7_05	593 876	C	93	G	93	PFE0705c	Helicase, belonging to UvrD family, putative	1786	596	CAA	Q	GAA	E	CAA	Q
Pf3D7_06	572 486	A	93	T	93	PFF0670w	Transcription factor with AP2 domain(s), putative	6353	2118	AAA	K	ATA	I	AAA	K
Pf3D7_10	1228 345	T	93	C	93	PF10_0294	RNA helicase, putative	746	249	CTC	L	CCC	P	CTC	L

SNPs were identified relative to the parental Dd2 genome sequence. Silent mutations and those occurring in highly polymorphic surface antigens were filtered from the dataset.

Abbreviations: a.a., amino acid; BRD6323R, BRD6323-resistant clone; ML238R, ML238-resistant clone; PCR, polymerase chain reaction; SNPs, single-nucleotide polymorphisms.

^a Whole-genome sequencing result.

^b Result from PCR resequencing of targeted region.