Figure 3.
SERPINH1 mutation causes a reduction in HSP47 and FKBP65 protein levels. (A–C) Protein levels for HSP47 and FKBP65 in HSP47M237T/M237T cells show a decrease in both proteins. (D and E) Transcriptional levels of HSP47 and FKBP65 do not change in HSP47M237T/M237T cells as measured by qRT-PCR. (F–H) Protein levels in FKBP10−/− cells show no change for HSP47 and absence of FKBP65. (I and J) Transcriptional levels for FKBP10−/− cells do not show changes in HSP47 and confirm lack of FKBP10 transcript.