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. 2014 Dec 8;24(7):1929–1944. doi: 10.1093/hmg/ddu609

Figure 2.

Figure 2.

Manganese-induced loss of viability in human neuroprogenitors is unaffected by mutant Huntingtin and not via apoptotic signaling. (A and B) The cell viability assay, CellTiterBlue, was performed on human neuroprogenitors (A) and mouse STHdh striatal cells (B). The signals were normalized by defining the vehicle-treated cells as 100% viability. Arrowheads denote concentrations chosen for the Pathscan array. (N = 6 for control and 5 for HD in human neuroprogenitors and N = 6 wells in mouse striatal cells). (B) Cleavage of PARP was quantified by the Pathscan Intracellular Signaling array from samples treated with manganese (N = 2 for human and 3 for mouse). (C) Cleavage of Caspase-3 was quantified by the Pathscan Intracellular Signaling array from samples treated with manganese (N = 2 for human and 3 for mouse). Values are relative to vehicle-treated samples. For genotype comparisons ***P < 0.001 by t-test. Bars, mean + SEM (A, C and D) and SD (B).