Table 1.
Antibodies
| Antigen | Species | Company | Catalog # | Model system | WB | ICW | IF |
|---|---|---|---|---|---|---|---|
| phos-ATM(Ser1981) | Mo | Cell Signaling | 4526 | Hu, Mo | 1:1000 | ||
| ATM | Rb | Cell Signaling | 2873 | Hu, Mo | 1:1000 | ||
| Caspase-3 | Rb | Cell Signaling | 9665 | Mo | 1:1000 | ||
| Huntingtin | Mo | Millipore | MAB2166 | Mo | 1:200 | ||
| Islet 1 & 2 | Mo | DSHB | 39.4D5 | Hu | 1:100 | ||
| Nanog | Gt | R&D Systems | AF1997 | Hu | 1:20 | ||
| Oct3/4 | Rb | Cell Signaling | 2750 | Hu | 1:200 | ||
| PAX6 | Rb | Covance (Biolegend) | PRB-278P | Hu | 1:100 | ||
| phospho-p53(Ser15) | Mo | Cell Signaling | 9286 | Hu, Mo | 1:1000 | 1:400 | |
| phospho-p53(Ser15) | Rb | Cell Signaling | 9284 | Hu, Mo | 1:1000 | 1:400 | |
| p53 | Mo | Santa Cruz | sc-126 | Hu | 1:1000 | ||
| p53 | Rb | Santa Cruz | sc-6243 | Mo | 1:1000 | ||
| phospho-H2A.X | Mo | EMD Millipore | 05-636 | Hu, Mo | 1:1000 | 1:400 | |
| phospho-CHK2(Thr68) | Rb | Cell Signaling | 2661 | Hu | 1:400 | ||
| SSEA3 | Rt | Millipore | MAB4303 | Hu | 1:500 | ||
| SSEA4 | Mo | DSHB | MC-813-70 | 1:500 | |||
| Anti Mouse IRDYE 800CW | Dk | LI-COR | 926–32 212 | 1:10 000 | 1:800 | ||
| Anti Rabbit IRDYE 800CW | Dk | LI-COR | 926–32 213 | 1:800 | |||
| Anti Rabbit IRDye 680LT | Dk | LI-COR | 926–68 023 | 1:10 000 | |||
| Anti (mo, rb, gt or rt) Dylight 488 or 549 | Dk | Jackson | 1:800 |
Antibodies used throughout this study are listed in the table with information about the species, vendor and model system used in as well as the dilution factor used for each assay type.