Table 2.

Primer sequences

Primer name	Gene	Sequence	Tm	Product size
AT83	FOXG1 F	CAACGGCATCTACGAGTTCA	59.9	57
AT84	FOXG1 R	TGCTTGTTCTCGCGGTAGTA	59.7
AT95	PAX6 F	CAGCTTCACCATGGCAAATA	59.7	77
AT96	PAX6 R	GCAGCATGCAGGAGTATGAG	59.6
AT103	Actin F	CTGTGGCATCCACGAAACTA	59.7	84
AT104	Actin R	AGCACTGTGTTGGCGTACAG	60.0
AT277	Islet-1 F	ACGGTGGCTTACAGGCTAAC	59.3	56
AT278	Islet-1 R	TTTCCAAGGTGGCTGGTAAC	60.0
1594	HTT CAG Repeat F	CCGCTCAGGTTCTGCTTTTA	60.5	278
1595	HTT CAG Repeat R	GGCTGAGGAAGCTGAGGAG	60.2	(19 CAG repeats)
Insertion1	WPRE F	CAGGCAACGTGGCGTGGTGT	70.0	265
Insertion2	WPRE R	GGACGTCCCGCGCAGAATCC	71.5
AT335	Mouse Actin F	CAGCCTTCCTTCTTGGGTAT	58.3	83
AT336	Mouse Actin R	CGGATGTCAACGTCACACTT	59.6
AT327	Mouse p21 F	GAGGCCCAGTACTTCCTCTG	58.9	78
AT328	Mouse p21 R	AGAGTGCAAGACAGCGACAA	59.8

Primers used for PCR and qRT-PCR are listed with both the ‘in-house’ primer name, gene name, sequence, melting temperature and the product size.