Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Bone. 2015 Jan 10;74:48–57. doi: 10.1016/j.bone.2015.01.001

Figure 4.

Figure 4

βEcd increases osteogenic differentiation of bone marrow stromal cells. Bone marrow cells were collected from long bones of male mice, two months of age and maintained in α-MEM with 10% FBS and antibiotics for four days. The adherent cells were collected and cultured in adipogenic or osteogenic media for 21 days. (A) Adipogenic differentiation measured by oil red staining and gene expressions related to adipogenesis (Cebp-α and Ppar-γ). (B) Osteogenesis measured the ratio of CFU-Ob/CFU-F and genes associated with osteoblast differentiation (Runx2 and Bglap1). Data are means ± SD. *: p<0.05 vs. control (PBS). All the studies were performed in triplicate.