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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Bone. 2015 Jan 10;74:48–57. doi: 10.1016/j.bone.2015.01.001

Figure 5.

Figure 5

Effects of βEcd on osteoblast autophagy in vitro. BMSC cells were obtained from dsRed-LC3 reporter male mice and cultured in osteogenic medium for 10 days before they were treated with PBS, Dexamethasone (Dex, 10-6 M), βEcd (10-7M) or combination of Dex + βEcd in serum-starved conditions for eight hours. (A and B) Representative images (A) and quantitation of (B) dsRed-LC dots (autophagosomes, white arrows) in BMSC grown in osteogenic medium for 7 days and after 8 hours serum starvation incubation with Dex or βEcd as indicated. More than 200 cells were analyzed per sample. All the studies were performed in triplicate.