Figure 2. ORC6 in the first zygotic cell cycle.

ICSI generated zygotes were fixed and stained for ORC6 at zygotic G1 (A), zygotic telophase (B), and 2-cell G1 (C) and visualized by confocal microscopy. Inset in (C) is the area in different confocal plane from the main image that contained the second nucleus. PB, second polar body. All images are shown at the same magnification, bar = 10 μm. An expanded version of this figure is shown in Suppl. Fig. 4.