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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Cell Biochem. 2015 May;116(5):778–786. doi: 10.1002/jcb.25033

Figure 5. ORC4 forms an ovoid sphere around the polar body chromatin at anaphase.

Figure 5

A GV oocyte was matured in vitro until it reached anaphase I and then fixed and stained for ORC4. The images are serial confocal planes starting at one chromosome set at anaphase I (A). The second set of chromosomes appears in (B). ORC4 begins as a small oval shape (B and C) then becomes a ring (D and E) then becomes a solid oval, again (F to G), indicating that ORC4 surrounds only one set of chromosomes as an ovoid sphere at anaphase I. All images are shown at the same magnification, bar = 10 μm.