Skip to main content
. Author manuscript; available in PMC: 2016 Mar 13.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Feb 13;458(3):626–631. doi: 10.1016/j.bbrc.2015.01.158

Figure 2. Isoaspartate protein damage in cultured hepatocytes, and rat liver cytosolic proteins.

Figure 2

Figure 2

(A) Hepatocytes cultured from control or ethanol-fed rats were treated with exogenous betaine, tubercidin, or adenosine, and the levels of isoaspartate damage quantified. (B) Liver cytosolic proteins from control (C) or ethanol (E) fed rats were methylated by PIMT using 3H-SAM. Radiolabelled proteins were resolved by 1D PAGE and visualised by autoradiography. Increased methylation of proteins from ethanol-fed rats was evident at molecular weights of ~75-80 kDa, ~95-100 kDa, and ~155-160 kDa. Profiles of radiolabelled proteins are shown from 3 different pairs of control and ethanol pair-fed animals.