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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: J Immunol. 2015 Feb 11;194(6):2635–2642. doi: 10.4049/jimmunol.1402261

FIGURE 5.

FIGURE 5

T cell activation is normal in Mir182−/− mice. (A) Sorted naïve splenic SP T cells were isolated from indicated mice and stimulated with plate-bound anti-CD3 (1μg/mL), soluble anti-CD28 (4μg/mL) and soluble IL-2 (1U/mL) and TaqMan qPCR was used to monitor expression of miR-182. (B, C) Sorted naïve splenic SP T cells were isolated from indicated mice and stimulated with plate-bound anti-CD3 (10, 1, or 0.2 μg/mL), soluble anti-CD28 (2 μg/mL) and soluble IL-2 (50, 25, 10, or 1 U/mL). Bar graphs depict % viability for CD4+/CD8+ SP T cells over stimulation conditions used. (D) Histogram overlay depicts representative CellTrace dilution profiles of CD4+ SP T cells stimulated with anti-CD3 (1 μg/mL), anti-CD28 (2 μg/mL) and IL-2 (1 U/mL). Representative proliferation index for same stimulation conditions is shown. (E) Histogram overlay depicts representative CellTrace dilution profiles of CD8+ SP T cells stimulated with anti-CD3 (10 μg/mL), anti-CD28 (2 μg/mL) and IL-2 (10 U/mL). Representative proliferation index for CD8+ SP T cells stimulated with anti-CD3 (1 μg/mL), anti-CD28 (2 μg/mL) and IL-2 (25 U/mL) is shown. (A–E) n=3, *p<0.1 (t-test). (B–E) Data representative of 2 experiments.