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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: J Immunol. 2015 Feb 13;194(6):2776–2785. doi: 10.4049/jimmunol.1401611

Figure 2. 15d-PGJ2 inhibits caspase-1 autoproteolysis and cytokine maturation of multiple inflammasomes.

Figure 2

Balb/cJ BMDMs were primed with LPS (1 μg/mL, 2 h). Cells were then exposed to 15d-PGJ2 (50 μM, 30 min) followed by (A) LT (1 μg/mL), (B) nigericin (50 μM), or (C) FlaTox (1 μg/mL) for indicated times. Western blotting of cell lysates and culture supernatants was performed with antibodies against the p10 fragment of caspase-1 or IL-1β. Balb/cJ BMDMs were primed with LPS (1 μg/mL, 2 h). Cells were then treated with 15d-PGJ2 (50 μM, 30 min) followed by FlaTox (1 μg/mL) for indicated times. Western blotting of cell lysates and culture supernatants was performed with antibodies against IL-1β. The * indicates a cross-reactive protein band which acts as an internal equal loading control. Results shown in these Westerns are representative of between four and up to sixteen similar experiments per treatment.