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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: J Immunol. 2015 Feb 13;194(6):2776–2785. doi: 10.4049/jimmunol.1401611

Figure 6. 15d-PGJ2 inflammasome inhibition is independent of COX-1.

Figure 6

(A) BMDMs from Cox1−/− (on the C57BL/6NTacNlrp1bS/S background) mice were primed with LPS (1 μg/mL, 2h). Cells were then incubated with 15d-PGJ2 (50 μM, 30 min) followed by LT (1 μg/mL, 75 min). Cell lysates were analyzed for IL-1β maturation by Western blot. (B) COX-1−/− BMDMs were exposed to varying concentrations of 15d-PGJ2 for 30 min. Cells were challenged with 1 μg/mL LT and viability was measured by MTT staining at 1.5 h post-LT. Each condition was assayed in triplicate. Curves are representative of two or more similar experiments. The * indicates a cross-reactive protein band which acts as an internal equal loading control.