Effect of BSO plus UVA light followed by 24 h of normal culture, on the morphology of cultured human lens epithelial cells. Cells were exposed to either BSO alone (overnight) or BSO (overnight) + UVA light (1 h) at either 3% or 20% O2, cultured normally for 24 h, and photographed. Conditions for UVA light exposure are shown in Fig. 1. (a) Control cells cultured normally; (b) BSO alone (overnight) + 24 h normal culture; (c) BSO + UVA light (1 hr, 3% O2) + 24 h normal culture; (d) BSO + UVA light (1 hr, 20% O2) + 24 h normal culture. Note the lack of change in appearance after 24 h of culture for cells treated with BSO alone (b), BSO + UVA light at 3% O2 (c), and BSO + UVA light at 20% O2 (d). Each photograph is representative of 4-6 experiments.