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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cytokine. 2015 Jan 31;72(2):135–145. doi: 10.1016/j.cyto.2014.11.015

Figure 4.

Figure 4

The effects of MIF blockade on osteoclastogenesis and transcription factors. A: RANKL-induced osteoclastogenesis, measured by the presence of TRAP+ MNCs, in RAW264.7 cells treated with anti-MIF monoclonal antibody (α-MIF) (100 ng/ml) or control IgG (100 ng/ml). B: Images of TRAP+ MNCs formed in cultures described in A; bars =200μm. Original magnification x100. C: Effect of α-MIF antibody (α-MIF) or control IgG on NF-κB reporter activity in RANKL (100 ng/ml, 6h)-treated stably transfected RAW cells. D: as C, but using RAW264.7 cells stably transfected with luciferase NFAT reporter. All values were expressed as mean±SEM. Experimental results were pooled from 3 independent experiments. *p<0.05, **p<0.01, ***p<0.001 relative to control antibody treated cultures.