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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Synapse. 2015 Mar;69(3):148–165. doi: 10.1002/syn.21800

Figure 1. Angiotensin type 1A (AT1aR)-enhanced green fluorescent protein (EGFP)-labeled cells in the hypothalamic paraventricular nucleus (PVN).

Figure 1

A: Diagram of a coronal section of the mouse brain, showing a representative rostrocaudal level of the PVN containing AT1aR-EGFP [modified from Hof et al. (2000)]. B: Dense cluster of AT1aR-EGFP-containing cells in the PVN. Higher magnification (inset) shows that dendritic processes (arrow) of PVN neurons can be identified. C: Representative immunoelectron microscopic picture showing GluN1-silver-intensified immunogld (SIG) particles in an AT1aR-EGFP-labeled (immunoperoxidase) PVN dendrite. Immunoperoxidase labeling for AT1aR-EGFP (black arrow) was found throughout the cytoplasm of dendritic profiles. GluN1-SIG particles (black dots) were localized in the plasmalemma (thin magenta arrow), near the plasmalemma (magenta arrowhead), and in the cytoplasm (thick magenta arrow). 3V, third ventricle; AHN, anterior hypothalamic nucleus; CEAm, central nucleus of the amygdala, medial part; CTX, cortex; SFO, subfornical organ; VMH, ventromedial nucleus of the hypothalamus. Scale bars: B, C = 500 nm; Inset, 25 μm