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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Cancer Discov. 2015 Jan 8;5(3):316–331. doi: 10.1158/2159-8290.CD-14-0736

Figure 7. Aberrant cytokine production in CD45.2 mutant and CD45.1 non-mutant cells from JAK2V617F-diseased mice.

Figure 7

A, Schematic illustration of bone marrow transplantation experiments using Jak2V617;Vav-Cre knock-in mice (CD45.2) as donors. B, Single cell analysis of sorted mutant (CD45.2) and non-mutant (CD45.1) populations from Jak2V617F-diseased mice (J2VF) and transplanted healthy controls (Ctrl). Percentages of cytokine secreting cells are shown above. C, Cytokine expression levels of sorted CD45.1-positive and –negative MF and Vav-Cre-negative control cells relative to CD45.1 control cells. D, Pie charts displaying the percentage of sorted mutant (CD45.2) and non-mutant (CD45.1) secreting different numbers of cytokines (0,1,2,3,4,5+). E, Cytokine secretion data showing that 59.3% of human PMF cells (patient PMF#11, allele burden: 38%) secrete at least one cytokine. F, Pie charts depicting the percentage of human PMF cells and control cells secreting different numbers of cytokines (0,1,2,3,4+).