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. 2015 Feb 24;2015:150390. doi: 10.1155/2015/150390

Figure 5.

Figure 5

Expressions of HPV16E7 mRNA and protein in Hep-2 cells treated with HPV16E7 siRNA. (a) The expression levels of HPV16E7 mRNA were assessed by qRT-PCR (2−ΔΔCt method. 2−ΔΔCt indicated relative expression level in the treatment group, compared with that in the control group). (b) The protein level of HPV16E7 in the HPV16E7 siRNA group was significantly reduced, compared with the scramble control group (* P < 0.05). (c) The expression levels of HPV16E7 protein were detected by Western blotting. (d) Hep-2 cells were treated with HPV16E7 siRNA and proliferation was measured by CCK-8 assay at 24 and 72 h after treatment.