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. 2015 Mar 5;12:7. doi: 10.1186/s12987-015-0003-0

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© Furihata et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of different basal medium and supplement combinations on Na-F permeability in HBMEC/ciβ-based blood–brain barrier model. Four media types were prepared, where either CSC or EBM2 basal medium (indicated by “Basal”) was supplemented by either cbR or SQs (indicated by “Suppl”). Three days after cell seeding, the initial medium was changed to one of the above-mentioned media. The cells were cultured continuously for 12 days, after which permeability assay was performed. Na-F permeability (P_app, × 10⁻³ cm/min) was expressed as mean ± S.D. of three independent experiments. The asterisk indicates p < 0.05 compared to the value of CSC-cbR.