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. 2015 Mar 5;12:7. doi: 10.1186/s12987-015-0003-0

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© Furihata et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Retardation of HBMEC/ciβ migration by hydrocortisone. Migration abilities of HBMEC/ciβ cultured with CSC-cbR, CSC-free, or CSC-HC were examined by scratch assay. The effect of RU486 on cell morphology was also tested using cells cultured with CSC-HC. Wound width was determined under microscopic observation at 0, 6 and 12 hours after the scratch had been made. The experiments were repeated three times and the representative results are shown. In the right side, the relative value of wound width was calculated as the value obtained at time 0 (immediately after scratch) was set to 1, and each value is expressed as mean ± S.D. obtained from three independent experiments. The asterisk indicates p < 0.05 compared to the value of CSC-free.