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. 2015 Mar 5;12:7. doi: 10.1186/s12987-015-0003-0

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© Furihata et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Matrix metalloproteinase mRNA expression profile in HBMEC/ciβ cultured with each medium. MMP-1, -2, and -16 mRNA expression levels in cells cultured with CSC-cbR, CSC-free, or CSC-HC were examined by qPCR. The effect of a GR antagonist, RU486, on HC-mediated modulation of mRNA expression was also investigated. The inset of the figure of MMP-1 shows magnified results obtained from cells cultured with CSC-free and CSC-HC (in the absence or presence of RU486) (left, middle, and right bar, respectively). mRNA expression levels were calculated relative to the value obtained from cells of CSC-cbR (set as basal level =1). Each value in the above experiments is expressed as mean ± S.D. obtained from three independent experiments. The “-” symbol indicates that DMSO (0.1%) was added to the medium as a control. The asterisk indicates p < 0.05 compared to the value of CSC-free.