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. 2015 Mar 4;8:36. doi: 10.1186/s13068-015-0214-2

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© Zhang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Investigation of the efficiency and stringency of the ARAi system in C . cellulolyticum H10. (A) Determination of inducible expression of GusA in H10::pARA-GusA with X-gluc as a substrate. The blue colors of the reaction solutions indicate the induced expression of GusA. The numbered tubes contained: 1, negative control without crude enzyme sample; 2, H10::pARA-PpFbFPm; 3, H10::pARA-GusA without L-arabinose; 4 to 6, H10::pARA-GusA with 0.1, 1 and 10 g/L L-arabinose, respectively. (B) Determination of GusA activity during a time course (0 to 6 h) using MUG as a substrate. Then 1 g/L L-arabinose was used as the inducer. (C) Determination of the influence of inducer dosage (0 to 10 g/L) on GusA activity using MUG as a substrate. The induction time was 2 h.