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. 2015 Mar 4;8:36. doi: 10.1186/s13068-015-0214-2

Figure 6.

Figure 6

Schematic representation and PCR confirmation of H10Δ pyrF transformants carrying pARA-PyrF- mspI or pARA-PyrF- cipC before FOA screening. Green dashed boxes indicated that the developed arabinose-inducible expression system containing a P ptk promoter and an AraR expression cassette (Additional file 1). The transformants were inoculated and cultivated in antibiotic-free medium until mid-log phase, and 1 g/L L-arabinose was added for induction for 0, 1, 2, and 4 h. Primer sets Ccel2866-F/R and Ccel0728-F/R were used for PCR flanking the intron target sites for gene mspI and cipC, respectively. The asterisks indicate the PCR products of mutants containing the 0.9-kb intron sequence, and the bands of 300 to 400 bp marked by triangles indicate the PCR products of host strain H10ΔpyrF. M, DNA marker (from top to bottom, 5000, 3000, 2000, 1500, 1000, 800, 500, and 300 bp).