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. 2015 Mar 4;15:72. doi: 10.1186/s12870-015-0453-1

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© Santopolo et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DAG2 inactivation does not affect embryo development. Histochemical staining of DAG2:GUS during embryogenesis, in early globular, globular, heart, late heart, torpedo and mature embryo (A).Relative expression level of DAG2 in wild-type seeds at 13, 16 and 19 Days After Pollination (DAP), and in mature seeds (28 DAR). Relative expression levels were normalized with that of the UBQ10 gene, and are presented by the ratio of the corresponding mRNA level in mature seeds, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05) (B). Phenotypes of wild-type (a, c) and dag2 mutant (b, d) embryos, at globular (a, b) and heart stage (c, d) (C).