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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2014 Dec 16;80:59–66. doi: 10.1016/j.freeradbiomed.2014.12.012

Scheme 3.

Scheme 3

5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide (BMPO, A). Upon incubation with BMPO in the enzyme reaction mixture, hydroperoxyl radical (B) binds to the nitroxide ring yielding a persistent radical adduct (C). (Double Column Image)