Table 1.
Sequence of peptides in I-Ab tetramers Each amino acid sequence contained in an I-Ab tetramer used in this study is listed. Numbers 1 through 9 above the sequences indicate the nonamer core predicted by Zhu et al. (Zhu et al., 2003) and Liu et al. (Liu et al., 2002) for each peptide with putative TCR contacts at P2, 5, 7, and 8 indicated by asterisks at the top and I-Ab anchors at P1, 4, 6, 7, and 9 denoted with carets at the bottom. The # signs refer to peptides that were locked into the I-Ab molecule in the indicated register by a disulfide trap strategy (Stadinski et al., 2010). The ESAT6 peptide is from the ESAT6 protein of Mycobacterium tuberculosis (Reiley et al., 2010); the FliC, STM1540-3, and STM1540-1 peptides are from the flagellin or STM1540 proteins of Salmonella enterica serovar Typhimurium (Maybeno et al., 2012; McSorley et al., 2000); the OVA2C and OVA3C peptides are from chicken ovalbumin (Moon et al., 2011; Robertson et al., 2000); the RpIF, Aasf, and PmpG-1 peptides are from ribosomal protein L6, anti-anti-sigma factor, and polymorphic membrane protein G of Chlamydia muridarum (Li and McSorley, 2013); the LLO peptide is from the listeriolysin O protein of Listeria monocytogenes (Geginat et al., 2001; Pepper et al., 2011); the CTB peptide is from the cholera toxin B subunit of Vibrio cholerae (Cong et al., 1996); the GP66 peptide is from glycoprotein 1 of Lymphocytic choriomeningitis virus (Oxenius et al., 1995); the CD4Ag28m peptide is from a putative transmembrane protein of Toxoplasma gondii (Grover et al., 2012); the Derp1 peptide is from the Der fl protein of Dermatophagoides farinae (Hoyne et al., 1994); the MOG peptide is from mouse myelin oligodendrocyte glycoprotein (Mendel et al., 1995); the calnexin peptide is from the calnexin protein of Blastomyces dermatitidis; the Cda2 peptide is from the chitin deacetylase 2 protein of Cryptococcus neoformans; the eGFP peptide is from enhanced green fluorescent protein. The 2W (Rees et al., 1999), 3K (Rees et al., 1999), 2W109, and 1G1W (Chu et al., 2010) peptides are variants of the E alpha peptide from the I-E MHCII molecule (Murphy et al., 1992).
| Peptide | Position (P) 123456789 * * ** |
|---|---|
| ESAT6 | QQWNFAGIEAAASA |
| FliC | VQNRFNSAITNLGNT |
| STM1540-3# | VYYTTYAPQAT |
| STM1540-1# | YTTYAPQATSA |
| eGFP | HDFFKSAMPEGYVQE |
| OVA3C# | GHAAHAEINEA |
| RplF | FVSPAAHII |
| OVA2C# | QAVHAAHAEIN |
| 3K | EAQKAKANKAVDKA |
| Calnexin | LVVKNPAAHHAIS |
| AasF | VSSPAVQES |
| 1G1W | EAGGALANWAVDSA |
| LLO# | NEKYAQAYPNVS |
| Derp1 | CQIYPPNVNKI |
| Cda2 | HQYMTALSNEVVF |
| PmpG-1 | YVDPAAAGG |
| CTB | NNKTPHAIAAIS |
| GP66 | DIYKGVYQFKSV |
| 2W | EAWGALANWAVDSA |
| CD4Ag28m | VEIHRPVPGTA |
| 2W109# | EYWGPLPNWV |
| MOG# | GWYRSPFSRVVVHLY |