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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cancer Prev Res (Phila). 2015 Jan 20;8(3):249–257. doi: 10.1158/1940-6207.CAPR-14-0224

Figure 4.

Figure 4

(A, B) Binding assays were performed by incubating BPH-1, BCaPM-T10, or LNCaP lysate with (A) EGCG-Sepharose or (B) a C-terminal HSP90 antibody and Agarose beads after competition with excess ligand. (C) Lysate from HEK293 cells transfected with FLAG-tagged HSP90 constructs was used for EGCG-Sepharose binding assays. Membranes were cut horizontally to probe for different proteins and bound/total proteins were determined by WB. Experiments were repeated 3 times; representative results shown.