Figure 3.

A single dose of Cp40 at the beginning of hemodialysis is sufficient to prevent complement activation. (A) Hemodialysis was performed in cynomolgus monkeys for a period of four hours and blood samples were collected prior to, during, and after blood circulation as indicated. Cp40 was injected as an i.v. bolus of 2 mg/kg. (B) Blood samples were collected from untreated (black line) and Cp40-treated (gray line) monkeys and the activation of complement C3 was measured in plasma samples by ELISA, represented as a percentage of total C3 activation (%). The figures depict data from blood samples collected from the inflow sampler (Figure 1A). Similar results were obtained with samples collected from the outflow sampler. (C) Levels of Cp40 were measured by mass spectrometry in the plasma of monkeys treated with Cp40. Samples were collected from both samplers as indicated in Fig. 1A.