Table 2.

Criteria to differentiate between a CMA substrate and an e-MI substrate

Criteria	CMA	e-MI
Half-Life of the substrate	Long Half-life	Long Half-life
Substrate association with organelle	CMA active lysosomes	Late endosomes/MVB a
Interacts with hsc70 through KFERQ-like motif	Yes	Yes
Interacts with LAMP2A	Yes	No
Requires protein substrate unfolding	Yes	No
Lumenal delivery	Translocation across lysosomal membrane Reduced by LAMP2A KD b	Internalization in small vesicles Reduced by ESCRT c proteins KD

^aMultivesicular bodies;

^bknock-down,

^cendosomal sorting complexes required for transport