Figure 3.

Routes of infection. Zebrafish are infected with M. marinum at different time points and via different inoculation routes. Systemic infection is achieved by injection into the caudal vein at 28 hpf or inoculation via the duct of Cuvier in embryos at 2–3 dpf (Benard et al. 2012). Local injection routes are the hindbrain ventricle, muscle, notochord (Alibaud et al. 2011), or otic vesicle. In addition to intravenous injection at 24–28 hpf, yolk injection can be applied at the one- to four-cell stage in a high-throughput setting (Benard et al. 2012).