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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mitochondrion. 2015 Jan 6;21:1–10. doi: 10.1016/j.mito.2014.12.005

Figure 3. Assembly and activity of respiratory chain enzyme complexes on blue native polyacrylamide gel.

Figure 3

Blue Native polyacrylamide gel from a.) muscle, b.) liver and c.) heart tissue showing activity staining for complexes I, II, IV and V as indicated. A) Little complex I or IV are appreciated in muscle, with incomplete complex V assembly as evidenced by lower molecular weight bands but with preserved complex II. B) Increased activity of complex II and incomplete complex V assembly in liver. C) Low levels of complex I and IV, with incomplete complex V assembly in heart. Ctrl- control tissue, HSD10- patient tissue.