RT-qPCR relative quantification of pre-processed mitochondrial RNA transcripts of tRNA Proline (mt.tRNAPro), tRNA Valine (mt.tRNAVal), NADH dehydrogenase 5 and 6 (ND5, ND6), Cytochrome B (CYTB), Cytochrome C oxidase-I (COI), and ATP synthase 6 (ATPase6) from a.) muscle and b.) heart; comparison between control (Ctrl) and patient (HSD10) tissues. Primer pairs on either side of 5’ start site for each gene. P values as noted: *p<0.01, **p<0.0005, ***p<0.0001, NS- not significant.