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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mitochondrion. 2015 Jan 6;21:1–10. doi: 10.1016/j.mito.2014.12.005

Figure 9. Northern blot analysis of unprocessed mitochondrial tRNA in HSD10 patient heart.

Figure 9

Total RNA from heart tissue of four controls (Lanes 1–4) and HSD10 patient (Lane 5) was isolated and resolved on TBE-urea polyacrylamide gel and transferred to a Hybond N+ membrane and probed with an antisense oligonucleotide probe to tRNA-Met. A band of unprocessed tRNA present in the HSD10 heart sample is strongly increased over controls.