Fig 5. Top network functions affected in the EMT process of cholangiocarcinoma cells.

Two relevant networks were generated from EMT-modulated proteins according to the Ingenuity Pathway Knowledge Criteria. A. Cellular compromise, cellular function and maintenance (score = 45). Red, upregulated proteins; green, significantly downregulated proteins; white, proteins known to be in the network but not identified in this study. The color depth indicates the magnitude of the change in protein expression levels. The shape is indicative of the molecular class (i.e protein family). Lines connecting the molecules indicate molecular relationships. Dashed lines indicate indirect interactions, and solid lines indicate direct interactions. The arrow styles indicate specific molecular relationships and the directionality of the interaction. B. Network build up from the three most significant bio-functions (activation Z score >2 or <2): cell survival, synthesis of nitric oxide and migration of cells. The symbols nomenclature is shown in the lower panel.