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Frequencies of total Ig and Env-specific ASCs and memory B cells in the peripheral blood of HIV-1-infected individuals. Mature B cells were enriched from PBMCs and evaluated for total Ig (left y-axis) and HIV-1 gp140-specific ASC frequencies by ELISPOT (left y-axis), as well as for binding to HIV-1 gp140 probe by flow cytometry (right y-axis). Sample numbers refer to individuals described in Table I; analyses were performed on 7 individuals.
