Table 2.
Cytokine production after exposing PMBCs for 24 h to L. kefiri strains. Cytokines concentrations in culture cell supernatant (pg mL−1) were measured using Flow Human Th1/Th2 11plex FlowCytomix Kit (eBioscience). The results are expressed as mean ± SD of experiments performed with three different donors.
| L. kefiri | IL-1β | IL-6 | IL-8 | IL-10 | IFN-γ | TNF-α | IL-12p70 |
|---|---|---|---|---|---|---|---|
| CIDCA 8321 | 1294 ± 526 | 1552 ± 709 | 5771 ± 1284 | 205 ± 76 | 131 ± 22 | 10436 ± 3785 | 312 ± 72 |
| CIDCA 8325 | 2050 ± 75 | 2571 ± 94 | 4824 ± 531 | 313 ± 11 | 59 ± 38 | 16169 ± 45 | 572 ± 94 |
| CIDCA 8345 | 1655 ± 8 | 2033 ± 15 | 4399 ± 106 | 230 ± 3 | 85 ± 20 | 15368 ± 1075 | 449 ± 21 |
| CIDCA 8348 | 1936 ± 10 | 2719 ± 13 | 3855 ± 40 | 435 ± 90 | 83 ± 4 | 13551 ± 198 | 502 ± 121 |
| CIDCA 83115 | 1023 ± 60 | 1778 ± 12 | 3621 ± 34 | 192 ± 9 | 49 ± 2 | 8613 ± 500 | 738 ± 206 |
| CIDCA 83111 | 604 ± 83 | 2401 ± 81 | 3806 ± 167 | 253 ± 1 | 103 ± 23 | 9908 ± 175 | 815 ± 189 |
| CIDCA 83113 | 1148 ± 26 | 1722 ± 95 | 3920 ± 202 | 201 ± 11 | 53 ± 22 | 7514 ± 427 | 475 ± 59 |
| JCM 5818 | 591 ± 103 | 919 ± 40 | 4228 ± 12 | 84 ± 2 | 62 ± 13 | 6872 ± 1647 | 246 ± 94 |
| Nonstimulated PBMC | 35 ± 2 | 71 ± 6 | 418 ± 202 | 21 ± 1 | 15 ± 11 | 175 ± 5 | 41 ± 13 |